In Silico Sequence Analysis, Structure Prediction and Evaluation of Fatty Acid Synthase Protein (FAS) of Gallus gallus (Chicken) by Homology Modelling

FAS (Fatty acid synthase) an enzyme catalysing synthesis of fatty acid. The protein contains two subunits,namely A & B. Fatty acid synthase protein plays a key role in fat deposition in chicken as it was found that the protein is directly correlated with fat content of chicken. Administration of glucocorticoids changes the expression of FAS protein.3D structure of FAS protein was not available on RCSB Protein Databank. FAS protein of Human was available in the database which was selected as template for homology modelling.The present study deals with sequence analysis, secondary structure prediction and homology modelling of Fatty acid synthase of chicken. The secondary structure of protein shows that the protein is chiefly of α-helical structure followed by coils and extended strand. 3D structure of protein was predicted using SwissModel. The predicted structure was evaluated by Procheck, Errat, Ramachandran Plot, QMean, Z-Score using SAVES, an online structure evaluation server (http://services.mbi.ucla.edu/SAVES/). Keyword: Fatty acid synthase, secondary structure, 3D structure, Homology modelling